Chimeric oligos with RNA, DNA, PTO and 2'O-Methyl-RNA
The use of 2'O-Methyl-RNA in chimeric antisense oligos increases nuclease stability of the oligo.
Chimeric oligonucleotides consist of mixed DNA and RNA bases including methylated bases or phosphorthioate bounds (PTO) in various numbers and compositions.
O-Methyl-RNA / chimerics are delivered in 2ml screw cap tubes according to your specification:
- Hugh variety of RNA modifications
- HPLC purified
- Synthesis scales from 0.05 µmol up to 1.0 µmol
- Sequence lengths from 6 to 80 bases
- Quality control by OD measurement and MALDI-TOF MS
- Delivery occurs lyophilised in tube format
HPLC is required for purification of chimeric oligos. We recommend to perform a Na+ salt exchange with commercially available desalting columns (e.g. NAP or MicroSpin columns) before use in cells or live animals.
What you can expect from our chimeric oligos
Product specifications:
- HPLC purified
- Synthesis scales from 0.05 µmol up to 1.0 µmol
- Minimum yields in OD
- Sequence lengths from 6 to 80 bases
- Quality control by OD measurement and MALDI-TOF MS
- TAT: 3-4 weeks
- Delivery: lyophilised in 2ml screw cap tubes
|
Synthesis scale1 [µmol]
|
0.05
|
0.20
|
1.00
|
| Min. Yield [OD] |
0.5 |
2 |
2.5 |
| Length restriction [bases] |
6 - 80 |
6 - 80 |
6 - 80 |
To differentiate between 2'O-Methyl-RNA, PTO, RNA and DNA bases enter your sequence as follows on the order page:
- DNA: ACGT
- RNA: (ACGU)
- PTO: A*C*(G*U)
- 2'-O-Methyl-RNA: [2OMeA], [2OMeC], [2OMeG], [2OMeU]
1 The synthesis scale indicates the initial amount of 3'-bases
PTOs, the defense line against nucleases
Phosphorothioate (PTOs) are the most widely used nuclease resistant oligos for antisense applications.
In PTO oligos, a non-bridging oxygen is replaced by a sulfur atom. Therefore, PTOs are also known as "S-oligos". Phosphorothioate bounds can be introduced to an oligo
- at the 5'- or 3'-end
Inhibits exonuclease degradation
- Internally
Limit the attack by endonucleases
Good to know!
PTO oligos can show greater non-specific protein binding than unmodified phosphodiester (PO) oligos. They can lead to toxic effects or cause artefacts building when present in high concentrations.
These problems can be reduced or eliminated by using chimeric designs, which limit the number of phosphorothioate internucleoside linkages within the oligo. See chimeric oligos.
What you can expect from oligos with phosphorothioate
Product specifications:
- Synthesis scales from 0.01 to 10 µmol
- Purification options: HPSF, HPLC, PAGE
- Oligo lengths: 5-120 bases
- TAT: 3-4 weeks
| Purification option |
Synthesis scale1 [µmol] |
0.01 |
0.05 |
0.20 |
1.00 |
| HPSF |
Minimum yield [OD]2 |
2 |
4 |
8 |
20 |
| HPLC |
Minimum yield [OD]2 |
1 |
3 |
5 |
15 |
| PAGE |
Minimum yield [OD]2 |
- |
0.5 |
0.5 |
- |