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Frequently Asked Questions About Our Products And Services

I see unspecific bands in my PCR reaction. Do I need to gel purify my product?

If you want to employ one of the PCR primers in the sequencing reaction you must elute the product of interest from an agarose gel. Otherwise, a mixed sequence will be produced as the primer is most likely to bind both the specific and unspecific products. If you employ a specific nested primer in the sequencing reaction, it might work. However, producing only one product in the PCR reaction is the best guarantee for a good sequence. In almost all cases, improving the PCR conditions (specific primer design, higher annealing temperature and/or lower primer concentration) helps to avoid unspecific products.

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