For your convenience, please use our Oligo Analysis Tool or apply the formula below:

N [nmol] = OD x 100 / 1.54 x n_A + 0.75 x n_C + 1.17 x n_G + 0.92 x n_T

n = number of nucleosides of the type X


Example:
Sequence: GAA ATG AGT GCT CAT CAC TAC TTC CGC (27mer)

n_A=7; n_C=8; n_G=5; n_T=7
OD=12 (measured)

N [nmol] = 12 x 100 / 1.54 x 7 + 0.75 x 8 + 1.17 x 5 + 0.92 x 7
= 41.3