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Frequently Asked Questions About Our Products And Services


How do I calculate the molecular weight M of an oligonucleotide?

For your convenience, please use our Oligo Analysis Tool or apply the formula below:

MW [g/mol]= 249.23 x nA + 240.21 x nT + 265.23 x nG + 225.20 x nC + 63.98 x (s-1) + 2.02

n = number of nucleosides of the type X
s = number of all nucleosides per sequence


Example:
Sequence: GAA ATG AGT GCT CAT CAC TAC TTC CGC (27mer)

nA=7; nC=8; nG=5; nT=7

MW [g/mol]= 249.23 x 7 + 240.21 x 7 + 265.23 x 5 + 225.20 x 8+ 63.98 x (s-1) + 2.02 = 8219.33

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