The synthesis scale indicates the amount of starting material
(CPG) of the solid phase synthesis process.
Not to be confused with the synthesis yield, which is depending
on the coupling efficiency during synthesis.
The coupling efficiency again depends on raw material quality.
The individual sequence and length of an oligonucleotide also
influences the coupling efficiency and therefore the synthesis
yield as shown in the table below:
| |
Yield
CE 99.5% |
Yield
CE 99.0% |
Yield
C E 98.5% |
| 20mer |
91 |
83 |
75 |
| 30mer |
86 |
75 |
65 |
| 40mer |
82 |
68 |
55 |
| 60mer |
74 |
55 |
41 |
| 100mer |
61 |
37 |
22 |
Theoretical yield of full length product = coupling efficiency (CE)
(length of oligonucleotide-1)