Available non-fluorescent modifications and their structures
Our non fluorescent modifications are characterised by
- guaranteed yields
We guarantee minimum yields in OD.
- maximum flexibility
Benefit from HPLC purification options in 3 different synthesis scales.
- highest quality
OD measurement and MALDI-TOF MS for each oligo ensures highest quality.
5' Amino groups are often used for coupling the oligonucleotides to solid supports. Amino groups can also be used to attach secondary modifications such as fluorescent dyes or large molecules like digoxigenin.
Structure of 5' Amino C6
Structure of 5' Amino C12
Biotinylated oligonucleotides are known for their ability to bind to streptavidin. Biotin added at the 3' end can be used to block exonuclease digestion.
5' Biotin structure
Biotin-TEG allows for an increase in binding to the streptavidin deep-binding pocket due to the fact that it has the longest linker arm from the Biotin group (e.g. used for hybridisation studies).
Cholesterol is a hydrophobic molecule. Coupled at the 3’ end of an oligonucleotide cholesterol has been established as a reliable courier through membranes and facilitates uptake into cells.
3' Cholesterol structure
Digoxigenin is used primarily as a non isotopic label for oligonucleotides. Common applications include diagnostics, sequencing, and in-situ hybridisations.
Phosphorylation allows the oligonucleotides to be used as a substrate for DNA ligase. 3' modifications can be used to block further extension by DNA. Terminal phosphates are also useful for enabling the ligation of two individual oligonucleotides together.
What you can expect from our oligos with non fluorescent modifications
- Synthesis scales from 0.05 to 1.0 µmol
- Purification options: HPLC
- Sequence lengths: 5-80 bases
- TAT: 2-3 weeks(depend upon dyes and synthesis site within Eurofins group)
||Synthesis scale1 [µmol]
||Minimum yield [OD]2