The right answers to frequently asked questions
Find the answers to all our products and services by clicking the links below.
Where do I obtain my results?
All raw data (FAST-Q files) as well as the analysed and assembled data can be downloaded via your secure Ecom online account. A demo data report for a typical project can be found under here.
What starting material should I send and how much of it?
SARS-CoV-2 Total RNA Genome sequencing
- Type: total RNA
- Quantity: 100 ng per sample
- Concentration: min 5 ng/µl
- Dissolved in: RNase-, DNase- and protease-free molecular grade water
- Please find in our sample submission guide detailed information about required sample volumes and shipping instructions
Artic SARS-CoV-2 RNA-Seq
- Type: total RNA
- Quantity: When the viral RNA has been previously assessed using a qPCR assay, the CT value should be between 18–35. If the CT is between 12–15, then dilute the sample 100-fold in water; if it's between 15–18 then dilute 10-fold in water. This will reduce the likelihood of PCR-inhibition.
- Purity: OD 260/280: 1.8 - 2.0; OD 260/230: 2.0 - 2.2; RIN or RQI value ≥ 8
- Dissolved in: RNase-, DNase- and protease-free molecular grade water
- Please find in our sample submission guidedetailed information about required sample volumes and shipping instructions
Do you guarantee a certain output?
SARS-CoV-2 Genome Sequencing
We guarantee 5 million read pairs (+/- 10%) per package.
Artic SARS-CoV-2 RNA-Seq
We deliver on average 5 million read pairs (+/- 10%) per sample. If the viral load is good (see our starting material requirements), then this is more than sufficient to assembly the full genome sequence.
How do I choose between the two SARS-CoV-2 products?
Artic SARS-CoV-2 RNA-Seq
The ARTIC PCR approach is reducing the number of undesired, off-target reads for the host. If you aim for a full virus genome sequence and no additional information go for the Artic SARS-CoV-2 product.
You will not receive any information for the host/patient from the collected sample.
SARS-CoV-2 Genome Sequencing
If you aim to receive contextual meta-transcriptomic patient/oral flora information in addition to the viral genome/sequence information use this product.
Where do I send my samples?
Eurofins Genomics Europe Sequencing GmbH
Jakob-Stadler-Platz 7
78467 Konstanz
Germany