For INVIEW CRISPR Check, the wildtype amplicon size needs to be adapted to the length of InDels you want to be able to analyse and must allow merging of read pairs. Usually mutations introduced via the NHEJ pathway are in a range of 1-20 bp, but in order to not miss the larger ones, the wildtype amplicons must be in a rather narrow size range. Length requirements for two use cases and all product types are available in our sample preparation guide.